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Home >> Antibodies >> PGAM1 Antibody / Phosphoglycerate mutase 1

PGAM1 Antibody / Phosphoglycerate mutase 1 (RQ7939)

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  Catalog No Formulation Size Price (USD)  
Image RQ7939 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human HeLa cells with PGAM1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with PGAM1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human HepG2, 4) human A549, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with PGAM1 antibody. Predicted molecular weight ~29 kDa.
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with PGAM1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PGAM1 antibody.
Flow cytometry testing of fixed and permeabilized human 293T cells with PGAM1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PGAM1 antibody.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human HepG2, 4) human A549, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with PGAM1 antibody and DyLight 647 secondary. Predicted molecular weight ~29 kDa.
Immunofluorescent staining of FFPE human HeLa cells with PGAM1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human thyroid cancer tissue with PGAM1 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testis cancer tissue with PGAM1 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P18669
Localization Cytoplasmic, nuclear
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow Cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations This PGAM1 antibody is available for research use only.
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Description

PGAM1 (Phosphoglycerate mutase 1) is a glycolytic enzyme that catalyzes the reversible conversion of 3-phosphoglycerate to 2-phosphoglycerate. As part of the central glycolysis pathway, PGAM1 plays a critical role in cellular energy metabolism and the production of precursors for biosynthetic pathways. Because of its importance in glucose utilization, a PGAM1 antibody is frequently used to study energy metabolism and metabolic reprogramming in cells.

PGAM1 is expressed in most tissues, with particularly high levels in proliferating cells and metabolically active tissues. Beyond its classical metabolic role, PGAM1 has been linked to regulation of the pentose phosphate pathway, nucleotide biosynthesis, and redox homeostasis. Dysregulation of PGAM1 expression has been associated with cancer progression, where increased glycolysis supports rapid cell growth and survival. Employing a PGAM1 antibody allows researchers to explore both its enzymatic function and its role in disease-associated metabolic shifts.

Recent studies have highlighted PGAM1 as a potential therapeutic target in oncology, since inhibition of its activity can disrupt tumor metabolism and impair cell proliferation. Additionally, PGAM1 is a useful marker for studying metabolic adaptations under stress conditions, such as hypoxia. A PGAM1 antibody can be applied in assays including western blot, immunohistochemistry, and immunofluorescence to assess protein expression and localization in diverse biological samples.

NSJ Bioreagents provides a validated PGAM1 antibody designed for reliable and reproducible results. Selecting the right PGAM1 antibody ensures accurate detection in studies of glycolysis, cancer biology, and metabolic regulation.

Application Notes

Optimal dilution of the PGAM1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids Q43-K254) was used as the immunogen for the PGAM1 antibody.

Storage

After reconstitution, the PGAM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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