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Home >> Antibodies >> PAX5 Antibody

PAX5 Antibody [clone PCRP-PAX5-1B1] (V3906)

  Catalog No Formulation Size Price (USD)  
Image V3906-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 539
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V3906-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V3906SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
Microvalidated
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Western blot testing of human Raji cell lysate with PAX5 antibody (clone PCRP-PAX5-1B1). Expected molecular weight ~42 kDa.
IHC testing of human lymph node with PAX5 antibody (clone PCRP-PAX5-1B1). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PAX5 antibody (clone PCRP-PAX5-1B1). These results demonstrate the foremost specificity of the PCRP-PAX5-1B1 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free PAX5 antibody (clone PCRP-PAX5-1B1) as confirmation of integrity and purity.
Availability Discontinued
Species Reactivity Human; other species not tested.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name PCRP-PAX5-1B1
Purity Protein G affinity chromatography
UniProt Q02548
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western Bot : 2-4ug/ml
Limitations This PAX5 antibody is available for research use only.
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Description

The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PAX5 antibody to be titered up or down for optimal performance.

Immunogen

Full length human recombinant protein was used as the immunogen for this PAX5 antibody.

Storage

Store the PAX5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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