Pan-HLA Antibody (DP/DQ/DR) [clone HLA-Pan/2967R] (V7585)

Immunofluorescence staining of human Raji cells with Pan-HLA antibody (green, clone HLA-Pan/2967R) and Reddot nuclear stain (red).

Immunofluorescence staining of human Ramos cells with Pan-HLA antibody (green, clone HLA-Pan/2967R) and Reddot nuclear stain (red).

IHC testing of FFPE human tonsil tissue with recombinant Pan-HLA antibody (clone HLA-Pan/2967R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool prior to testing.

Flow cytometry testing of human Ramos cells with Pan-HLA antibody (clone HLA-Pan/2967R); Red=isotype control, Blue= Pan-HLA antibody.

Western blot testing of human Ramos lysate with Pan-HLA antibody (clone HLA-Pan/2967R). Expected molecular weight: 33-35 kDa.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Pan-HLA antibody (clone HLA-Pan/2967R). These results demonstrate the foremost specificity of the HLA-Pan/2967R mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant Pan-HLA antibody (clone HLA-Pan/2967R) as confirmation of integrity and purity.