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Home >> Antibodies >> NOTCH2 Antibody

NOTCH2 Antibody (RQ5542)

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  Catalog No Formulation Size Price (USD)  
Image RQ5542 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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IHC staining of FFPE human glioblastoma tissue with NOTCH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human glioblastoma tissue with NOTCH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with NOTCH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with NOTCH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with NOTCH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with NOTCH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human U-87 MG, 2) human 293T, 3) human MCF7 and 4) human SH-SY5Y cell lysate with NOTCH2 antibody. Predicted molecular weight ~265 kDa (full length) and ~110 kDa (intracellular/cytoplasmic domain).
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with NOTCH2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOTCH2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q04721
Localization Nuclear, cytoplasmic
Applications Western Blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/million cells
Immunohistochemistry (FFPE) : 2-5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This NOTCH2 antibody is available for research use only.
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Description

Neurogenic locus notch homolog protein 2 also known as notch 2 is a protein that in humans is encoded by the NOTCH2 gene. It is mapped to 1p12. This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the NOTCH2 antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids H2194-A2471) was used as the immunogen for the NOTCH2 antibody.

Storage

After reconstitution, the NOTCH2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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