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Home >> Antibodies >> NMDAR2A Antibody

NMDAR2A Antibody (R30238)

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Image R30238 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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IHC staining of FFPE rat brain tissue with NMDAR2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat brain tissue with NMDAR2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with NMDAR2A antibody. Predicted molecular weight ~165 kDa.
Availability Discontinued
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q12879
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations This NMDAR2A antibody is available for research use only.
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  • Applications : WB
    Reactivity : Human, Mouse, Rat

Description

N-methyl-D-aspartate receptor channel, subunit epsilon-1 (NMDAR2A or NR2A) is a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the NMDAR2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human NMDAR2A (DHTSDNPFLHSHRDDQR) was used as the immunogen for this NMDAR2A antibody.

Storage

After reconstitution, the NMDAR2A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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