NDUFS2 Antibody / NADH dehydrogenase ubiquinone iron-sulfur protein 2 [clone 30N44] (FY12621)

Immunohistochemical staining of NDUF-S2 using anti-NDUF-S2 antibody. NDUF-S2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUF-S2 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.

Western blot analysis of NDUFS2 using anti-NDUFS2 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human TE-1 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS2 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot probed with anti-NDUFS2 shows a strong band at ~43 kDa, lower than the predicted ~53 kDa, consistent with the mature mitochondrial form of NDUFS2 lacking its cleaved N-terminal targeting sequence.

Immunohistochemical staining of NDUF-S2 using anti-NDUF-S2 antibody. NDUF-S2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUF-S2 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.

Immunohistochemical staining of NDUF-S2 using anti-NDUF-S2 antibody. NDUF-S2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUF-S2 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.