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Home >> Antibodies >> NCL Antibody / Nucleolin

NCL Antibody / Nucleolin [clone 29N05] (RQ8805)

  Catalog No Formulation Size Price (USD)  
Image RQ8805 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 439
Microvalidated Recrabbitmono
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Western blot testing of human HeLa cell lysate with NCL antibody. Predicted molecular weight ~77 kDa, commonly observed at 100~110 kDa.
Availability 1-3 days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name 29N05
Purity Affinity chromatography
UniProt P19338
Applications Western Blot : 1:500-1:2000
Limitations This NCL antibody is available for research use only.
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Description

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. [UniProt]

Application Notes

Optimal dilution of the NCL antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human Nucleolin protein was used as the immunogen for the NCL antibody.

Storage

Store the NCL antibody at -20oC.

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