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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
MYLK3 Antibody / Myosin light chain kinase 3 (FY13284)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY13284 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
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MYLK3 antibody targets Myosin light chain kinase 3, a cardiac-specific serine/threonine kinase that phosphorylates the regulatory light chain of myosin II to modulate cardiac muscle contraction. The MYLK3 gene encodes cardiac myosin light chain kinase (cMLCK), which plays a pivotal role in sarcomere assembly, contractile force generation, and maintenance of cardiac output. cMLCK phosphorylates myosin regulatory light chain (MLC2v), enhancing the interaction between actin and myosin filaments to promote efficient contraction of cardiomyocytes.
MYLK3 expression is restricted mainly to cardiac muscle tissue, distinguishing it from other members of the myosin light chain kinase family such as MYLK and MYLK2, which function in smooth and skeletal muscle, respectively. Located on chromosome 16q23.1, the MYLK3 gene is activated during cardiac differentiation and is transcriptionally regulated by cardiac transcription factors including NKX2-5 and GATA4. Its kinase domain shares structural homology with other Ca2+/calmodulin-dependent kinases but exhibits distinct substrate specificity suited to cardiac myosin regulation.
Functional studies demonstrate that MYLK3 enhances cardiac contractility by maintaining phosphorylation of ventricular myosin light chain 2 (MLC2v). Loss-of-function mutations or reduced expression of MYLK3 have been linked to dilated cardiomyopathy and impaired sarcomere organization. Overexpression of MYLK3 increases calcium sensitivity and promotes sarcomere maturation during cardiomyocyte development, emphasizing its importance in cardiac physiology. In experimental models, altered MYLK3 signaling can influence cardiac hypertrophy, mechanical stress responses, and recovery following myocardial injury.
Immunostaining using MYLK3 antibody reveals strong cytoplasmic localization in cardiac muscle fibers, particularly in ventricular tissue. The antibody is widely applied to research focused on cardiac muscle contractility, signal transduction, and kinase-mediated phosphorylation events. MYLK3 antibody from NSJ Bioreagents provides a reliable tool for investigating cardiac-specific myosin regulation, sarcomere dynamics, and disease mechanisms associated with cardiomyopathies.
Optimal dilution of the MYLK3 antibody should be determined by the researcher.
E.coli-derived human MYLK3 recombinant protein (Position: E34-K812) was used as the immunogen for the MYLK3 antibody.
After reconstitution, the MYLK3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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