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Home >> Antibodies >> MIS18A Antibody / Protein Mis18-alpha / C21orf45

MIS18A Antibody / Protein Mis18-alpha / C21orf45 (RQ8414)

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  Catalog No Formulation Size Price (USD)  
Image RQ8414 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human HeLa cells with MIS18A antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with MIS18A antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) 293T and 2) HeLa cell lysate with MIS18A antibody. Predicted molecular weight ~26 kDa.
Flow cytometry testing of fixed and permeabilized human HEL cells with MIS18A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MIS18A antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9NYP9
Localization Nuclear
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This MIS18A antibody is available for research use only.
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Description

Protein Mis18-alpha is a protein that in humans is encoded by the MIS18A gene. C21orf45, also named as MIS18A, C21orf46 and FASP1, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. C21orf45 has dimer (~52 kDa) and heterodimer(~105 kDa) forms.

Application Notes

Optimal dilution of the MIS18A antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (M1-D233) was used as the immunogen for the MIS18A antibody.

Storage

After reconstitution, the MIS18A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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