Melanoma gp100 Antibody / PMEL17 [clone PMEL/2039] (V3929)

IHC testing of human skin with Melanoma gp100 antibody (clone PMEL/2039). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of human melanoma with Melanoma gp100 antibody (clone PMEL/2039). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Melanoma gp100 antibody (clone PMEL/2039). These results demonstrate the foremost specificity of the PMEL/2039 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Melanoma gp100 antibody (clone PMEL/2039) as confirmation of integrity and purity.

Western blot testing of human COLO-38 cell lysate with PMEL17 antibody (clone PMEL/2039). The ~100 kDa glycosylated PMEL precursor is proteolytically cleaved into an ~60-64 kDa M-alpha fragment and an ~26 kDa M-beta fragment. The M-alpha fragment is subsequently processed into ~34-38 kDa and ~26 kDa fragments that assemble into the fibrillar matrix of melanosomes.