- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Melanoma gp100 Antibody / PMEL17 [clone MSSG95-2] (V3916)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V3916-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 539 | |
|
|
V3916-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 249 | |
|
|
V3916SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 539 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | MSSG95-2 |
| Purity | Protein G affinity chromatography |
| UniProt | P40967 |
| Localization | Cytoplasmic |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This Melanoma gp100 antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Melanoma gp100 antibody (clone MSSG95-2) detects PMEL, also known as Melanocyte protein PMEL17 or premelanosome protein, a pigment cell-specific structural glycoprotein that supports melanin biosynthesis. The UniProt recommended name is Melanocyte protein PMEL (PMEL). gp100 is a defining component of the melanosome matrix, where it assembles into fibrillar scaffolds that direct pigment polymerization and organelle maturation in melanocytes and melanoma cells. Its restricted expression pattern and biochemical function make it a reliable marker for melanocytic differentiation and melanoma identification.
The PMEL gene resides on chromosome 12q13.2 and encodes a type I transmembrane protein that undergoes extensive post-translational modification and proteolytic processing. Following synthesis, PMEL passes through the endoplasmic reticulum and Golgi apparatus, where it becomes glycosylated and cleaved into functional fragments. These fragments accumulate within early-stage melanosomes (stage II) to form amyloid-like fibrils, which serve as the foundational framework for melanin deposition. Proper PMEL assembly is essential for melanosome integrity, while defective processing results in disorganized pigment granules and altered melanin storage.
PMEL expression is tightly controlled by the transcription factor MITF (microphthalmia-associated transcription factor), a central regulator of melanocyte lineage specification. gp100 is expressed in normal pigment cells, retinal pigment epithelium, and most melanomas. In the skin, it is localized to dendritic extensions of melanocytes that facilitate pigment transfer to neighboring keratinocytes. Within the eye, it contributes to pigmentation of the retinal pigment epithelium and visual homeostasis. Because PMEL is expressed exclusively in pigment cell lineages, it serves as a powerful biomarker for melanoma detection and classification.
Clone MSSG95-2 is a monoclonal antibody developed for the detection of gp100 in mammalian tissues and cultured cells. It recognizes PMEL protein in melanocytes and melanoma models, producing consistent labeling of pigment organelles. The antibody can be applied to evaluate melanosome maturation, melanocytic phenotype, and pigmentation patterns in tumor and developmental studies. Its high specificity for PMEL17 makes it valuable for distinguishing melanocytic origin in research and diagnostic applications.
Beyond pigmentation biology, gp100 has clinical significance as a melanoma-associated antigen that elicits T-cell immune responses. gp100-derived peptides have been studied for their potential to stimulate antitumor immunity and are used in immunotherapy research targeting melanoma. Detection of PMEL expression assists in correlating antigen presentation, differentiation status, and tumor behavior, offering insights into melanoma progression and treatment response.
Melanoma gp100 antibody (clone MSSG95-2) is suitable for identifying PMEL expression in melanoma tissue, pigment cell cultures, or developmental models of melanogenesis. NSJ Bioreagents provides Melanoma gp100 antibody (clone MSSG95-2) validated for use in relevant research applications supporting studies in pigment cell biology, melanoma immunology, and melanosome organization.
The stated application concentrations are suggested starting points. Titration of the Melanoma gp100 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 376-502 from the human protein was used as the immunogen for the Melanoma gp100 antibody.
Store the Melanoma gp100 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
Citations(5)
info@nsjbio.com