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Home >> Antibodies >> MAPE Antibody / PRAME

MAPE Antibody / PRAME (F55105)

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  Catalog No Formulation Size Price (USD)  
Image F55105-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 429
Image
F55105-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 199
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IHC staining of FFPE human testis tissue with MAPE antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC staining of FFPE human testis tissue with MAPE antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HeLa, 2) K562, 3) HEK293, 4) testis, 5) brain and 6) kidney tissue lysate with MAPE antibody. Predicted molecular weight ~58 kDa.
Flow cytometry testing of fixed and permeabilized human HeLa cells with MAPE antibody; Blue=isotype control, Green= MAPE antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P78395
Localization Cytoplasm, Nucleus, Cell Membrane
Applications Western Blot : 1:1000-1:2000
Immunohistochemistry (FFPE) : 1:50-1:100
Flow Cytometry : 1:25 per million cells in 0.1ml
Limitations This MAPE antibody is available for research use only.
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Description

MAPE (Melanoma antigen preferentially expressed in tumors), also called PRAME (Preferentially expressed antigen of melanoma), is a protein that is preferentially expressed in tumors, particularly in melanoma. It plays a vital role in regulating cell proliferation, migration, and invasion, making it a promising target for therapeutic interventions. Studies have shown that the overexpression of MAPE protein is associated with aggressive melanoma subtypes, indicating its potential as a prognostic biomarker in melanoma patients.

Application Notes

The stated application concentrations are suggested starting points. Titration of the MAPE antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 476-502 from the human protein was used as the immunogen for the MAPE antibody.

Storage

Aliquot the MAPE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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