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Home >> Antibodies >> MAP4K5 Antibody

MAP4K5 Antibody (RQ6106)

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  Catalog No Formulation Size Price (USD)  
Image RQ6106 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human A431 cells with MAP4K5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A431 cells with MAP4K5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human ovarian cancer with MAP4K5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Jurkat, 2) K562, 3) HepG2 and 4) HEK293 cell lysate with MAP4K5 antibody. Predicted molecular weight ~95 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q9Y4K4
Applications Western Blot : 1-2ug/ml
Immunofluorescence : 5ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations This MAP4K5 antibody is available for research use only.
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  • Applications : WB, FACS, ELISA
    Reactivity : Human

Description

Mitogen-activated protein kinase kinase kinase kinase 5 is an enzyme that in humans is encoded by the MAP4K5 gene. This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Application Notes

Optimal dilution of the MAP4K5 antibody should be determined by the researcher.

Immunogen

Amino acids AADILRRNPQQDYELVQR from the human protein were used as the immunogen for the MAP4K5 antibody.

Storage

After reconstitution, the MAP4K5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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