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Home >> Antibodies >> LDOC1 Antibody / Leucine zipper protein down-regulated in cancer cells

LDOC1 Antibody / Leucine zipper protein down-regulated in cancer cells (RQ8485)

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  Catalog No Formulation Size Price (USD)  
Image RQ8485 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human HeLa cells with LDOC1 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with LDOC1 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human HeLa cell lysate with LDOC1 antibody. Predicted molecular weight ~17 kDa.
Flow cytometry testing of fixed and permeabilized human U-251 cells with LDOC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDOC1 antibody.
Availability 1-3 days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O95751
Localization Nuclear
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This LDOC1 antibody is available for research use only.
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Description

Protein LDOC1 is a protein that in humans is encoded by the LDOC1 gene. The protein encoded by this gene contains a leucine zipper-like motif and a proline-rich region that shares marked similarity with an SH3-binding domain. The protein localizes to the nucleus and is down-regulated in some cancer cell lines. It is thought to regulate the transcriptional response mediated by the nuclear factor kappa B (NF-kappaB). The gene has been proposed as a tumor suppressor gene whose protein product may have an important role in the development and/or progression of some cancers.

Application Notes

Optimal dilution of the LDOC1 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids H10-E140) was used as the immunogen for the LDOC1 antibody.

Storage

After reconstitution, the LDOC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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