ITPA Antibody | Inosine triphosphate pyrophosphatase

	Catalog No	Formulation	Size	Price (USD)
	RQ8653	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	439
Formulation

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IHC staining of FFPE human pancreas ductal adenocarcinoma tissue with ITPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human testicular seminoma tissue with ITPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon adenocarcinoma tissue with ITPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with ITPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with ITPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human urothelial carcinoma tissue with ITPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human glioblastoma tissue with ITPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat colon tissue with ITPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human intestinal cancer tissue with ITPA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Western blot testing of 1) human K562, 2) human HepG2, 3) human HeLa, 4) human A549, 5) rat liver and 6) mouse liver tissue lysate with ITPA antibody. Predicted molecular weight ~21 kDa.

Flow cytometry testing of fixed and permeabilized human K562 cells with ITPA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ITPA antibody.

Availability	1-3 days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity chromatography
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q9BY32
Localization	Cytoplasm
Applications	Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Flow Cytometry : 1-3ug/million cells ELISA : 0.1-0.5ug/ml
Limitations	This ITPA antibody is available for research use only.
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Description

Inosine triphosphate pyrophosphatase is an enzyme that in humans is encoded by the ITPA gene, by the rdgB gene in bacteria E.coli and the HAM1 gene in yeast S. cerevisiae; the protein is also encoded by some RNA viruses of the Potyviridae family. This gene encodes an inosine triphosphate pyrophosphohydrolase. The encoded protein hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. This protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency which causes an accumulation of ITP in red blood cells. Alternate splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the ITPA antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids M1-A194) was used as the immunogen for the ITPA antibody.

Storage

After reconstitution, the ITPA Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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ITPA Antibody / Inosine triphosphate pyrophosphatase / ITPase (RQ8653)

Description

Application Notes

Immunogen

Storage

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Area of Research

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Protocols

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