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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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Four structurally related IL-1 receptor ligands have been described. These include three agonists designated IL-1a, IL-1b and IL-1g/IL-18 and a specific receptor antagonist, IL-1Ra. IL-1a and IL-1b play critical roles in the regulation of the immune response and inflammation, serving as activators of T and B lymphocytes and NK (natural killer) cells. IL-18 (also referred to as IL-1g) has been shown to augment the secretion of IFN-g from T lymphocytes and increase NK cell activity in spleen cells. IL-18 exhibits 19% and 12% identity with IL-1a and IL-1b respectively over the 12 beta-strands of the beta-trefoil fold domain, which is a signature feature of the IL-1 family. The unusual leader sequence of IL-18 may be analogous to the IL-1b pro-domain which must be cleaved by the serine protease ICE for optimal secretion and biological activity. Originally described as IGIF (IFN-g-inducing factor), IL-18 is induced by mouse liver subsequent to challenge with lipopolysaccharide (LPS).
Optimal dilution of the Interleukin 18 antibody should be determined by the researcher.
A recombinant fragment (within amino acids 1-193) of human IL-18 protein was used as the immunogen for the Interleukin 18 antibody.
Aliquot the Interleukin 18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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