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Home >> Antibodies >> ICAM5 Antibody / Intercellular adhesion molecule 5

ICAM5 Antibody / Intercellular adhesion molecule 5 (RQ8634)

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  Catalog No Formulation Size Price (USD)  
Image RQ8634 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Flow cytometry testing of fixed and permeabilized human HeLa cells with ICAM5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ICAM5 antibody.
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Flow cytometry testing of fixed and permeabilized human HeLa cells with ICAM5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ICAM5 antibody.
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with ICAM5 antibody. Predicted molecular weight ~97 kDa but may be observed at higher molecular weights due to glycosylation.
Availability 1-3 days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9UMF0
Applications Western Blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This ICAM5 antibody is available for research use only.
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Description

Intercellular adhesion molecule 5 is a protein that in humans is encoded by the ICAM5 gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.

Application Notes

Optimal dilution of the ICAM5 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids R430-G682) was used as the immunogen for the ICAM5 antibody.

Storage

After reconstitution, the ICAM5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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