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Home >> Antibodies >> Histone H1.10 Antibody / H1-10

Histone H1.10 Antibody / H1-10 (RQ8788)

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  Catalog No Formulation Size Price (USD)  
Image RQ8788 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human HeLa cells with Histone H1.10 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with Histone H1.10 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) 293T and 3) U-2 OS cell lysate with Histone H1.10 antibody. Predicted molecular weight ~22 kDa.
Availability 1-3 days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q92522
Localization Nuclear
Applications Western Blot : 1-2ug/ml
Immunofluorescence : 5ug/ml
ELISA : 0.1-0.5ug/ml
Limitations This Histone H1.10 antibody is available for research use only.
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Description

Histone H1x is a protein that in humans is encoded by the H1FX gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H1 family.

Application Notes

Optimal dilution of the Histone H1.10 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids M1-K106) was used as the immunogen for the Histone H1.10 antibody.

Storage

After reconstitution, the Histone H1.10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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