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Home >> Antibodies >> Hexokinase 1 Antibody / HK1

Hexokinase 1 Antibody / HK1 [clone HK1/9510] (V5732)

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  Catalog No Formulation Size Price (USD)  
Image V5732-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5732-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5732SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human brain tissue with Hexokinase 1 antibody (clone HK1/9510). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human brain tissue with Hexokinase 1 antibody (clone HK1/9510). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human brain tissue lysate with Hexokinase 1 antibody (clone HK1/9510). Predicted molecular weight ~120 kDa.
SDS-PAGE analysis of purified, BSA-free Hexokinase 1 antibody (clone HK1/9510) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name HK1/9510
Purity Protein G affinity
UniProt P19367
Applications Western Blot : 2-4ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Hexokinase 1 antibody is available for research use only.
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Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

Application Notes

Optimal dilution of the Hexokinase 1 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1-200 from human HK1 protein was used as the immunogen for the Hexokinase 1 antibody.

Storage

Aliquot the Hexokinase 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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