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Home >> Antibodies >> HDDC2 Antibody / HD domain-containing protein 2

HDDC2 Antibody / HD domain-containing protein 2 (RQ8764)

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  Catalog No Formulation Size Price (USD)  
Image RQ8764 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human U-2 OS cells with HDDC2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with HDDC2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) K562, 3) MCF7 and 4) PC-3 cell lysate with HDDC2 antibody. Predicted molecular weight ~23 kDa.
Flow cytometry testing of fixed and permeabilized human SiHa cells with HDDC2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HDDC2 antibody.
Availability 1-3 days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q7Z4H3
Applications Western Blot : 1-2ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This HDDC2 antibody is available for research use only.
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Description

HDDC2 (HD Domain Containing 2) is located on human chromosome 6q22.31. The HDDC2 protein is ubiquitously expressed in adrenal, brain and other tissues. Structurally, the encoded protein is reported to be 27160 Da in mass. The HDDC2 (also known as C6orf74; CGI-130; NS5ATP2; dJ167O5.2) gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, rice, frog, etc. 239 organisms have orthologs with human gene HDDC2. HDDC2 catalyzes the dephosphorylation of the nucleoside 5'-monophosphates deoxyadenosine monophosphate (dAMP), deoxycytidine monophosphate (dCMP), deoxyguanosine monophosphate (dGMP) and deoxythymidine monophosphate (dTMP).

Application Notes

Optimal dilution of the HDDC2 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids A7-S204) was used as the immunogen for the HDDC2 antibody.

Storage

After reconstitution, the HDDC2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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