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Home >> Antibodies >> GFAP Antibody / Glial Fibrillary Acidic Protein

GFAP Antibody / Glial Fibrillary Acidic Protein [clone GFAP/9424] (V5715)

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  Catalog No Formulation Size Price (USD)  
Image V5715-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5715-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5715SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/9424). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/9424). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human brain tissue with GFAP antibody (clone GFAP/9424). Predicted molecular weight ~50 kDa.
SDS-PAGE analysis of purified, BSA-free GFAP antibody (clone GFAP/9424) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name GFAP/9424
Purity Protein G affinity
UniProt P14136
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This GFAP antibody is available for research use only.
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Description

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]

Application Notes

Optimal dilution of the GFAP antibody should be determined by the researcher.

Immunogen

A recombinant full-length human GFAP protein was used as the immunogen for the GFAP antibody.

Storage

Aliquot the GFAP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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