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Home >> Antibodies >> GATA4 Antibody

GATA4 Antibody (F43474)

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  Catalog No Formulation Size Price (USD)  
Image F43474-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 429
Image
F43474-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 199
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GATA4 antibody flow cytometric analysis of HepG2 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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GATA4 antibody flow cytometric analysis of HepG2 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot analysis of GATA4 antibody and CEM lysate. Predicted molecular weight: 42-50 kDa.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat, Xenopus
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P43694
Applications Western Blot : 1:1000
Flow Cytometry : 1:10-1:50
Limitations This GATA4 antibody is available for research use only.
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Description

GATA4 is a transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. [UniProt]

Application Notes

Titration of the GATA4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 298-328 from the human protein was used as the immunogen for this GATA4 antibody.

Storage

Aliquot the GATA4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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