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Home >> Antibodies >> Fox2 Antibody / RBM9

Fox2 Antibody / RBM9 (RQ5548)

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Image RQ5548 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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IHC staining of FFPE mouse brain tissue with Fox2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse brain tissue with Fox2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain cortex tissue with Fox2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat hippocampus tissue with Fox2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE endometrial cancer tissue with Fox2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE mouse brain tissue with Fox2 antibody (green). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat brain tissue with Fox2 antibody (green). HIER: steam section in pH8 EDTA buffer for 20 min.
Western blot testing of 1) human U-2 OS, 2) human Caco-2, 3) human A431, 4) human U-87 MG, 5) rat C6 and 6) mouse NIH 3T3 cell lysate with Fox2 antibody. Expected molecular weight ~41 kDa.
Immunofluorescent staining of FFPE human U-2 OS cells with Fox antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O43251
Localization Nuclear
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Fox2 antibody is available for research use only.
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  • Applications : WB, IHC-P, IF, FACS, Direct ELISA
    Reactivity : Human, Mouse, Rat

Description

RNA binding motif protein 9 (RBM9), also known as Rbfox2, is a protein which in humans is encoded by the RBM9 gene. It is mapped to 22q12.3. This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the Fox2 antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids M1-Q81) was used as the immunogen for the Fox2 antibody.

Storage

After reconstitution, the Fox2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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