• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> FBXO32 Antibody / F-box only protein 32 / Atrogin 1

FBXO32 Antibody / F-box only protein 32 / Atrogin 1 (RQ8589)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image RQ8589 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
Bulk quote request
Immunofluorescent staining of FFPE human HeLa cells with FBXO32 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
›
Immunofluorescent staining of FFPE human HeLa cells with FBXO32 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) mouse heart and 2) mouse skeletal muscle tissue lysate with FBXO32 antibody. Predicted molecular weight ~42 kDa.
Flow cytometry testing of fixed and permeabilized human PC-3 cells with FBXO32 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FBXO32 antibody.
Availability 1-3 days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q969P5
Localization Cytoplasm, Nucleus
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This FBXO32 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

F-box only protein 32, also known as MAFbx, for Muscle Atrophy F-box gene, and Atrogin-1, is a protein that in humans is encoded by the FBXO32 gene. This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Notes

Optimal dilution of the FBXO32 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids Q6-F355) was used as the immunogen for the FBXO32 antibody.

Storage

After reconstitution, the FBXO32 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all FBXO32 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.