ELF3 Antibody / E74-like factor 3 (FY12381)

Immunoprecipitation of ELF3 in whole cell lysate. Western blot analysis of ELF3 using anti-ELF3 antibody. Lane 1: whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-ELF3 antibody in whole cell lysate, Lane 3: anti-ELF3 antibody (2ug) + whole cell lysate (500ug). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ELF3 antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. ELF3 (~41 kDa predicted) was detected as bands at ~50 kDa, and ~100 kDa. The ~50 kDa form corresponds to phosphorylated/SUMO-modified ELF3, while the ~100 kDa species likely represents a dimeric or complex-associated form, consistent with published reports.

Western blot analysis of ELF3 using anti-ELF3 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELF3 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. ELF3 (~41 kDa predicted) was detected as bands at ~42 kDa, ~50 kDa, and ~100 kDa. The ~50 kDa form corresponds to phosphorylated/SUMO-modified ELF3, while the ~100 kDa species likely represents a dimeric or complex-associated form, consistent with published reports.