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Home >> Antibodies >> Dihydrodiol dehydrogenase 1 Antibody / DDH1 / AKR1C1

Dihydrodiol dehydrogenase 1 Antibody / DDH1 / AKR1C1 [clone AKR1C1/9318] (V5685)

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  Catalog No Formulation Size Price (USD)  
Image V5685-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5685-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5685SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human hepatocellular carcinoma tissue with Dihydrodiol dehydrogenase 1 antibody (clone AKR1C1/9318). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human hepatocellular carcinoma tissue with Dihydrodiol dehydrogenase 1 antibody (clone AKR1C1/9318). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human A549 cell lysate with Dihydrodiol dehydrogenase 1 antibody (clone AKR1C1/9318). Predicted molecular weight ~37 kDa.
Western blot testing of human HepG2 cell lysate with Dihydrodiol dehydrogenase 1 antibody (clone AKR1C1/9318). Predicted molecular weight ~37 kDa.
SDS-PAGE analysis of purified, BSA-free Dihydrodiol dehydrogenase 1 antibody (clone AKR1C1/9318) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name AKR1C1/9318
Purity Protein G affinity
UniProt Q04828
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This Dihydrodiol dehydrogenase 1 antibody is available for research use only.
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Description

DDH1 / AKR1C1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone.

Application Notes

Optimal dilution of the Dihydrodiol dehydrogenase 1 antibody should be determined by the researcher.

Immunogen

A recombinant human full-length AKR1C1 protein was used as the immunogen for the Dihydrodiol dehydrogenase 1 antibody.

Storage

Aliquot the Dihydrodiol dehydrogenase 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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