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Home >> Antibodies >> DAZAP1 Antibody

DAZAP1 Antibody (RQ5253)

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Image RQ5253 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
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IHC staining of FFPE rat testis tissue with DAZAP1 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
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IHC staining of FFPE rat testis tissue with DAZAP1 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE mouse testis tissue with DAZAP1 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
Western blot testing of 1) human AGS, 2) human GES-1, 3) human HGC-27, 4) human HeLa, 5) human Jurkat, 6) human 293T, 7) rat PC-12 and 8) mouse NIH 3T3 cell lysate with DAZAP1 antibody. Predicted molecular weight ~43 kDa.
Availability 1-2 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q96EP5
Localization Cytoplasmic, Nuclear
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This DAZAP1 antibody is available for research use only.
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Description

DAZ-associated protein 1 is a protein that in humans is encoded by the DAZAP1 gene. It is mapped to19p13.3. In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene.

Application Notes

Optimal dilution of the DAZAP1 antibody should be determined by the researcher.

Immunogen

Amino acids M1-E189 from the human protein were used as the immunogen for the DAZAP1 antibody.

Storage

Store the DAZAP1 antibody at -20oC.

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