Crk-like protein Antibody / CrkL (RQ7731)

Description

Crk-like protein (CrkL) is an adaptor protein belonging to the Crk family of signaling molecules. It contains one SH2 domain and two SH3 domains, allowing it to interact with a wide range of tyrosine-phosphorylated proteins and downstream effectors. Through these interactions, CrkL regulates pathways involved in cytoskeletal organization, cell migration, adhesion, proliferation, and survival. CrkL is expressed in many tissues but is particularly important in hematopoietic and immune cell signaling.

Aberrant regulation of CrkL has been linked to various human diseases, most notably chronic myeloid leukemia (CML), where it serves as a major substrate of the BCR-ABL fusion protein. Elevated phosphorylation and altered signaling through CrkL contribute to leukemogenesis and resistance to therapy. CrkL has also been implicated in solid tumors, immune regulation, and developmental processes, highlighting its role as a central node in intracellular signaling networks.

The Crk-like protein antibody is a powerful tool for investigating signaling pathways associated with cell migration, oncogenesis, and immune regulation. Researchers use the Crk-like protein antibody in western blotting, immunoprecipitation, and immunofluorescence to detect expression and phosphorylation states of CrkL. With proven sensitivity and specificity, the Crk-like protein antibody enables accurate analysis of intracellular signaling dynamics in both normal physiology and disease models.

Application Notes

Optimal dilution of the Crk-like protein antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-H290) was used as the immunogen for the Crk-like protein antibody.

Storage

After reconstitution, the Crk-like protein antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.