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Home >> Antibodies >> Creatine Kinase M Antibody / CKM

Creatine Kinase M Antibody / CKM [clone CKMM/3341] (V8504)

  Catalog No Formulation Size Price (USD)  
Image V8504-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 539
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V8504-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 249
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V8504SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human skeletal muscle tissue with Creatine Kinase M antibody (clone CKMM/3341). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skeletal muscle tissue with Creatine Kinase M antibody (clone CKMM/3341). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human skeletal muscle tissue lysate with Creatine Kinase M antibody. Predicted molecular weight ~43 kDa.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Creatine Kinase M antibody (clone CKMM/3341). These results demonstrate the foremost specificity of the CKMM/3341 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name CKMM/3341
Purity Protein G affinity chromatography
UniProt P06732
Localization Cytoplasm
Applications Western Blot : 2-4ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Creatine Kinase M antibody is available for research use only.
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Description

T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells bind the homologous T cell receptors CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and CD28 and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counter receptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell.

Application Notes

Optimal dilution of the Creatine Kinase M antibody should be determined by the researcher.

Immunogen

Recombinant full-length CKM protein was used as the immunogen for the Creatine Kinase M antibody.

Storage

Store the Creatine Kinase M antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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