CD86 Antibody BU63 | V2056 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2056-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	539
Formulation
	V2056-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	249
Formulation
	V2056SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	539
Formulation

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Flow cytometry testing of PFA-fixed human Ramos cells with CD86 antibody (clone BU63); Red=isotype control, Blue= CD86 antibody.

FACS staining of human PBMCs using CD86 antibody (red, clone BU63) and isotype control (green).

Immunofluorescent staining of PFA-fixed human Ramos cells with CD86 antibody (clone BU63, green) and Reddot nuclear stain (red).

SDS-PAGE analysis of purified, BSA-free CD86 antibody (clone BU63) as confirmation of integrity and purity.

Species Reactivity	Human
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	BU63
Purity	Protein G affinity chromatography
Gene ID	942
Localization	Cytoplasmic, membrane
Applications	Flow Cytometry : 1-2ug/million cells Immunofluorescence : 1-3ug/ml
Limitations	This CD86 antibody is available for research use only.
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Description

This antibody recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by Bcell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in Tcell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD86 antibody to be titered up or down for optimal performance.