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Home >> Antibodies >> CD44 Antibody / H-CAM

CD44 Antibody / H-CAM [clone HCAM/6779] (V5493)

  Catalog No Formulation Size Price (USD)  
Image V5493-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5493-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5493SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human tonsil tissue with CD44v6 antibody (clone HCAM/6779). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD44v6 antibody (clone HCAM/6779) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name HCAM/6779
Purity Protein A/G affinity
UniProt P16070
Localization Cell membrane, cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CD44 antibody is available for research use only.
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Description

This antibody recognizes an epitope encoded by exon v3 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA.The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.

Application Notes

Optimal dilution of the CD44 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human CD44 protein was used as the immunogen for the CD44 antibody.

Storage

Aliquot the CD44 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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