- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD3e Antibody [clone OKT3] (V8204)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V8204-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 539 | |
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V8204-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 249 | |
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V8204SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 539 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2a, kappa |
| Clone Name | OKT3 |
| Purity | Protein G affinity chromatography |
| UniProt | P07766 |
| Localization | Cell surface, cytoplasmic |
| Applications | Flow Cytometry : 1-2ug/10^6 cells Immunohistochemistry (acetone Fixed Frozen Sections) : Immunofluorescence : 1-2ug/ml Functional Studies (order BSA/azide-free Format) : |
| Limitations | This CD3e antibody is available for research use only. |
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CD3e antibody detects the epsilon chain of the CD3 complex, encoded by the CD3E gene. CD3 epsilon is a core component of the T-cell receptor-CD3 complex, which is essential for antigen recognition and T-cell activation. Because CD3 is expressed on nearly all mature T cells, CD3e antibody is a critical reagent in immunology, oncology, and translational medicine.
CD3 epsilon contains extracellular immunoglobulin-like domains, a single transmembrane region, and a cytoplasmic tail with immunoreceptor tyrosine-based activation motifs (ITAMs). These motifs initiate intracellular signaling upon antigen-MHC engagement. Phosphorylation of the ITAMs recruits kinases such as Lck and Zap70, activating downstream cascades including MAPK, NF-κB, and NFAT. This signaling drives proliferation, effector differentiation, and cytokine secretion, establishing CD3 epsilon as a cornerstone of adaptive immunity.
The CD3e antibody clone OKT3 is one of the most historically important monoclonal antibodies in immunology. Clone OKT3 was the first monoclonal antibody approved for clinical use, originally employed to prevent transplant rejection by modulating T-cell activity. In research, it has been extensively cited in peer-reviewed studies examining T-cell signaling, receptor clustering, and immune checkpoint regulation. Its reproducibility makes it suitable for flow cytometry, immunohistochemistry, and in vitro functional assays.
Research using clone OKT3 has demonstrated how CD3 epsilon signaling integrates with costimulatory and inhibitory pathways to shape T-cell responses. The antibody has been applied in mechanistic studies of thymocyte development, immune synapse formation, and effector T-cell expansion. In oncology, clone OKT3 has supported work on bispecific antibodies and CAR T-cell strategies, where CD3 engagement drives targeted cytotoxicity against cancer cells. Its historical significance and broad application highlight its continuing relevance to both basic and translational research.
NSJ Bioreagents supplies this CD3e antibody to support immunology, oncology, and therapeutic development. Alternate terms include CD3 epsilon chain antibody, T-cell receptor CD3 complex antibody, T-cell surface glycoprotein CD3e antibody, TCR-associated protein epsilon antibody, and T-cell activation marker antibody.
Optimal dilution of the CD3e antibody should be determined by the researcher.
Human PBMC were used as the immunogen for the CD3e antibody.
Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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