Cd163 Antibody for Research | RQ8935 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ8935	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	439
Formulation

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Flow cytometry testing of fixed mouse RAW264.7 cells with Cd163 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd163 antibody.

IHC staining of FFPE rat thymus tissue with Cd163 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) rat spleen, 2) rat PC-12, 3) mouse thymus and 4) mouse RAW264.7 cell lysate with Cd163 antibody. Predicted molecular weight ~130 kDa but may be observed at higher molecular weights due to glycosylation.

Availability	1-2 business days
Species Reactivity	Mouse, Rat
Format	Antigen affinity purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q2VLH6
Localization	Cell surface, cytoplasmic
Applications	Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml ELISA : 0.1-0.5ug/ml Flow Cytometry : 1-3ug/million cells
Limitations	This Cd163 antibody is available for research use only.
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Description

Cd163 is a type I transmembrane glycoprotein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is highly expressed on cells of the monocyte/macrophage lineage, where it functions as a hemoglobin-haptoglobin complex receptor. By mediating clearance of hemoglobin from sites of tissue damage or intravascular hemolysis, Cd163 plays a central role in protecting tissues from oxidative stress and inflammation. A Cd163 antibody is frequently used to study macrophage biology, immune regulation, and tissue remodeling in both mouse and rat models.

Cd163 expression is strongly associated with alternatively activated (M2) macrophages, which are involved in anti-inflammatory processes, tissue repair, and immunosuppression. This makes Cd163 a valuable marker for identifying macrophage polarization states. Employing a Cd163 antibody allows researchers to investigate the dynamics of macrophage activation in response to injury, infection, or disease progression.

Beyond its role in hemoglobin clearance, Cd163 has broader immunomodulatory functions. Engagement of Cd163 can trigger anti-inflammatory signaling cascades and promote the release of heme oxygenase-1, further contributing to cytoprotection. Dysregulated Cd163 expression has been implicated in conditions such as sepsis, atherosclerosis, and chronic inflammatory diseases. In cancer, Cd163-positive macrophages are often linked to tumor-associated macrophages (TAMs), which can support tumor growth and immune evasion. A Cd163 antibody is therefore a powerful tool in both basic immunology and translational research.

NSJ Bioreagents provides a high-quality Cd163 antibody validated for use in western blot, immunohistochemistry, and flow cytometry. Choosing a Cd163 antibody from NSJ Bioreagents ensures reliable performance and reproducibility in studies of macrophage biology, immune responses, and inflammation in rodent models.

Application Notes

Optimal dilution of the Cd163 antibody should be determined by the researcher.

Immunogen

Amino acids N122-H1039 from the mouse protein were used as the immunogen for the Cd163 antibody.

Storage

After reconstitution, the Cd163 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Cd163 Antibody (RQ8935)

Description

Application Notes

Immunogen

Storage

Area of Research

Product Type

Protocols

Contact Us

NSJ Bioreagents