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Home >> Antibodies >> Arginase 1 Antibody / ARG1

Arginase 1 Antibody / ARG1 [clone ARG1/9448] (V5755)

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  Catalog No Formulation Size Price (USD)  
Image V5755-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5755-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5755SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human hepatocellular carcinoma tissue with Arginase 1 antibody (clone ARG1/9448). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human hepatocellular carcinoma tissue with Arginase 1 antibody (clone ARG1/9448). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human liver tissue lysate with Arginase 1 antibody (clone ARG1/9447). Predicted molecular weight ~35 kDa.
SDS-PAGE analysis of purified, BSA-free Arginase 1 antibody (clone ARG1/9448) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name ARG1/9448
Purity Protein G affinity
UniProt P05089
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This Arginase 1 antibody is available for research use only.
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Description

Arginase belongs to the ureohydrolase family of enzymes. Arginase catalyzes the fifth and final step in the urea cycle, a series of biochemical reactions in mammals during which the body disposes of harmful ammonia. Specifically, arginase converts L-arginine into L-ornithine and urea. [Wiki]

Application Notes

Optimal dilution of the Arginase 1 antibody should be determined by the researcher.

Immunogen

A recombinant human ARG1 partial protein (within amino acids 11-97) was used as the immunogen for the Arginase 1 antibody.

Storage

Aliquot the Arginase 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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