Anti-EpCAM Antibody / Extracellular domain [clone EGP40/1372] (V3525)

Description

Anti-EpCAM antibody is a well established reagent for investigating epithelial cell adhesion molecule, also known as EpCAM. This transmembrane glycoprotein is expressed on the surface of most epithelial cells and is overexpressed in many carcinomas. EpCAM mediates cell cell adhesion, regulates signaling pathways, and influences cell proliferation and differentiation. Its widespread presence in epithelial tissues and cancers has made it an important focus in both biology and pathology.

EpCAM contributes to intercellular adhesion by forming homophilic interactions at epithelial junctions. Beyond structural roles, it functions as a signaling regulator, with its intracellular domain influencing transcriptional activity linked to proliferation. Dysregulated expression of EpCAM is common in epithelial tumors, where it supports growth, migration, and metastasis. Because of this, EpCAM has been investigated as both a biomarker and a therapeutic target.

The Anti-EpCAM antibody clone EGP40/1372 provides specific recognition of this glycoprotein. Clone EGP40/1372 has been applied in cancer biology to track EpCAM expression in colorectal, breast, ovarian, and pancreatic carcinomas. It has also been used in stem cell research, where EpCAM marks epithelial progenitors and cancer stem cell populations. Its reproducibility makes it valuable for experiments requiring reliable detection of EpCAM across tissues.

Research involving EpCAM has expanded understanding of tumor biology and cell adhesion. Studies using clone EGP40/1372 have highlighted the role of EpCAM in regulating proliferation and invasion, clarifying why it is consistently overexpressed in carcinomas. Beyond oncology, EpCAM serves as a marker for epithelial cells in development and regenerative medicine, where it supports identification of progenitor populations.

NSJ Bioreagents offers this Anti-EpCAM antibody to support investigations into epithelial biology, cancer progression, and stem cell research. Alternate names include epithelial cell adhesion molecule antibody, ESA antibody, TACSTD1 antibody, and 323A3 12 antibody, which reflect the varied nomenclature encountered in scientific studies.

Application Notes

Titering of the anti-EpCAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the anti-EpCAM antibody.

Storage

Store the anti-EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).