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banner Search results for SPINK5

SPINK5 Antibody / Serine protease inhibitor Kazal-type 5 (clone 29S14)

Catalog No : RQ8860
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Purified
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Western blot testing of mouse lung tissue lysate with SPINK5 antibody. Predicted molecular weight ~121 kDa.
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SPINK5 Antibody / Serine protease inhibitor Kazal-type 5

Catalog No : FY12394
Applications : WB, ELISA
Reactivity : Mouse, Rat
Format : Lyophilized
Western blot analysis of Spink5 using anti-Spink5 antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat stomach tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse stomach tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPINK5 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. Spink5 (~121 kDa predicted) was detected at ~105 kDa, consistent with the partially processed, glycosylated precursor described in keratinocyte and epithelial studies.
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