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banner Search results for SIPA1

SIPA1 Antibody / Signal-induced proliferation-associated 1

Catalog No : FY12879
Applications : WB, ICC/IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunofluorescent staining of SIPA1 using anti-SIPA1 antibody (green) and anti-Beta Tubulin antibody (red). SIPA1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SIPA1 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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SIPA1 Antibody

Catalog No : R33174
Applications : WB, ELISA (peptide)
Reactivity : Human
Format : Antigen affinity purified
Western blot testing of human EBV-immortilized lymphoblastoid lysate with SIPA1 antibody at 1ug/ml, with (B) and without (A) immunizing peptide. Predicted molecular weight: ~112kDa but routinely observed at ~130kDa. It is observed here at ~150kDa.
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