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MELTF Antibody / Melanotransferrin

Catalog No : FY12944
Applications : WB, IHC, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of MELTF using anti-MELTF antibody. Lane 1: rat skin tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse skin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MELTF antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A single band is detected at ~90 kDa in rat and mouse samples, running above the ~80 kDa prediction. The higher migration is consistent with the mature, heavily N-glycosylated and GPI-anchored forms of melanotransferrin commonly observed in rodent tissues.
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