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INAVA Antibody / Innate immunity activator

Catalog No : FY12179
Applications : WB, ELISA
Reactivity : Human
Format : Lyophilized
Western blot analysis of INAVA using anti-INAVA antibody. Lane 1: human Hacat whole cell lysates, Lane 2: human whole cell lysates, Lane 3: human whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INAVA antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected band size for INAVA is at 73/64 kDa (two isoforms).
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