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  • Tel: 858.663.9055
  • Email: info@nsjbio.com
banner Search results for Eukaryotic Translation Initiation Factor

Phospho-eIF4EBP1 (Thr46) Antibody / Eukaryotic translation initiation factor 4E binding protein 1 (clone 32E52)

Catalog No : FY12420
Applications : WB, IHC
Reactivity : Human, Mouse
Format : Liquid
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Western blot analysis of 4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) using anti-Phospho-eIF4EBP1 (Thr46) antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for 4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) at approximately 18 kDa. The expected molecular weight of 4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) is at 18 kDa.
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EIF1 Antibody / Eukaryotic translation initiation factor 1

Catalog No : RQ7993
Applications : WB, IHC-P, IF, FACS, Direct ELISA
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
IHC staining of FFPE human placental tissue with Eukaryotic translation initiation factor 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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EIF1 Antibody / Eukaryotic translation initiation factor 1

Catalog No : F53885
Applications : WB
Reactivity : Human, Mouse
Pred. Reactivity :Bovine
Format : Antigen affinity purified
Western blot testing of 1) human HL-60 and 2) mouse Neuro-2a cell lysate with EIF1 antibody at 1:2000. Predicted molecular weight: 13 kDa.
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EIF1 Antibody / Eukaryotic translation initiation factor 1

Catalog No : F53886
Applications : WB
Reactivity : Human, Mouse
Pred. Reactivity :Bovine, Pig, Chicken
Format : Antigen affinity purified
Western blot testing of mouse Neuro-2a cell lysate with EIF1A antibody at 1:2000. Predicted molecular weight: 13 kDa.
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EIF5 Antibody / Eukaryotic translation initiation factor 5

Catalog No : RQ8103
Applications : WB, IHC-P, FACS, Direct ELISA
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
IHC staining of FFPE human esophageal squamous carcinoma tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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EIF5 Antibody / Eukaryotic translation initiation factor 5 (clone 2F13C4)

Catalog No : RQ6953
Applications : WB, IF, FACS
Reactivity : Human
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human PC-3 cells with EIF5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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EIF6 Antibody / Eukaryotic Translation Initiation Factor 6

Catalog No : R32150
Applications : WB, IHC-P, ICC
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Western blot testing of 1) rat heart, 2) rat liver, 3) mouse liver, 4) human placenta, 5) COLO320 and 6) HeLa lysate with Eukaryotic Translation Initiation Factor 6 antibody. Expected molecular weight ~27 kDa.
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EIF6 Antibody / Eukaryotic Translation Initiation Factor 6

Catalog No : R31140
Applications : WB, IHC-P, IHC-F
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Western blot testing of EIF6 antbody; Lane 1: rat liver; 2: (r) kidney; 3: human COLO320; 4: (h) SW620; 5: (h) HeLa; 6: (h) 293T; 7: mouse HEPA cell lysate. Expected molecular weight ~27 kDa.
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EIF3A Antibody / Eukaryotic translation initiation factor 3 subunit A (clone 31E29)

Catalog No : FY12073
Applications : WB, IHC, ICC, IF
Reactivity : Human, Mouse, Rat
Format : Liquid
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Immunofluorescent analysis using the EIF3A antibody (green) at 1:50 dilution.
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EIF3B Antibody / Eukaryotic translation initiation factor 3 subunit B

Catalog No : RQ8651
Applications : WB, IHC-P, IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
IHC staining of FFPE human colon tissue with EIF3B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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