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  • separatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
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Signal Transduction Antibodies

Home >> Antibodies >> Signal Transduction Antibodies

Signal Transduction Antibodies

NSJ Bioreagents offers the following antibodies in the area of Signal Transduction:
Found all antibodies, displaying 10921 to 10923
CLIC4 Antibody / Chloride intracellular channel protein 4 (clone 30C24)
Catalog No : FY12005
Applications : WB, IHC, ICC, IF, IP, FACS
Reactivity : Human, Mouse, Rat
Format : Liquid
Recrabbitmono
Western blot analysis of CLIC4 using anti-CLIC4 antibody. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIC4 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for CLIC4 at approximately 29 kDa. The expected band size for CLIC4 is at 29 kDa.
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LAT Antibody / Linker for activation of T-cells family member 1
Catalog No : FY12034
Applications : ELISA, WB, FACS
Reactivity : Human
Format : Lyophilized
Flow Cytometry analysis of HEL cells using anti-LAT antibody. Overlay histogram showing HEL cells stained with (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LAT antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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KEAP1 Antibody / Kelch-like ECH-associated protein 1
Catalog No : FY12106
Applications : WB, ICC, IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
IF analysis of KEAP1 using anti-KEAP1 antibody (green). KEAP1 was detected in an immunocytochemical section of cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-KEAP1 antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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