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Antibodies

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PCCA Antibody / Propionyl-CoA carboxylase alpha Perilipin 2 Antibody / Antibodies
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Antibody Listing

Found all antibodies, displaying 3361 to 3370

ZYX Antibody / Zyxin (clone AFOG-26)

Catalog No : FY12408
Applications : WB, IHC, ICC, IF, IP, FACS
Reactivity : Human, Mouse, Rat
Format : Liquid
Western blot analysis of Zyxin using anti-Zyxin antibody. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Zyxin antibody at 1:1000 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Zyxin (ZYX, ~61 kDa predicted) was detected at ~75-80 kDa, consistent with its phosphorylated and isoform-dependent mobility reported in the literature. Lower bands (~35-45 kDa) likely represent proteolytic fragments generated during turnover or stress responses.
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